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Manuals: Cryo-Electron Microscopy Facility - UT Southwestern, Dallas, Texas Manuals Reducing MRC Files Size During SP Data Collection, Oct. 19, 2021 Krios Embedded Start Up S.O.P., Nov. 25, 2019 Updated Manual for 'On The Fly Processing', Nov. 22, 2019 Protocol Low-dose serial EM setup for UTSW Talos

Training: Nuclear Magnetic Resonance Core - UT Southwestern, Dallas, Texas Training All users must complete the MR safety I training and sign and submit the MR Screening Form and the Biochemistry NMR Safety Form before they are permitted to enter the magnet rooms or request NMR training.

Pixel Size and Dose information 1 Set up parameters (ss, C2/illumination area) precisely for data collection: Find a target/hole -> On Gatan (K3) PC -> DM -> take an image Image Properties -> Pixel Size Dose information Find an empty area or undock grid (use vacuum) -

Deltavision Deconvolution Microscopes: Quantitative Light Microscopy Core Facility - UT Southwestern, Dallas, Texas Deltavision Deconvolution Microscopes (K1.228, NL5.120R) Inverted wide-field epifluorescence microscopes engineered for high fidelity image acquisition from live or fixed samples up to

Zeiss SteREO Discovery: Quantitative Light Microscopy Core Facility - UT Southwestern, Dallas, Texas Zeiss SteREO Discovery (NL7.110M) Zeiss motorized stereo microscope with filters for GFP, RFP, and CY5 GFP LP available for simultaneous GFP and transmitted-light imaging Automated collection of z-stacks

Intellectual Property - Histo Pathology Core - EDU - UT Southwestern, Dallas, Texas Intellectual Property The Histo Pathology Core has developed and/or advanced the intellectual properties listed below.

Gene therapy has shown great promise as a method for treating a wide range of genetic diseases, and AAV vectors are among the most commonly used delivery mechanisms for these therapies due to their low risk of immunogenicity and their ability to target various tissue types.

Zeiss LSM880 Airyscan: Quantitative Light Microscopy Core Facility - UT Southwestern, Dallas, Texas Zeiss LSM880 Airyscan (K1.224) For conventional and superresolution confocal microscopy of immunofluorescent cell cultures and tissue sections. 405, 458, 488, 514, 561, and 633 nm laser lines 32-channel

Zeiss AxioObserver: Quantitative Light Microscopy Core Facility - UT Southwestern, Dallas, Texas Zeiss AxioObserver (K1.222) Zeiss Axiovert 100M with motorized focus drive DIC and Phase contrast optics at 10, 20, 40, 63 and 100x Fluorescence filter sets for DAPI, Texas Red, FITC, CY5 and CY7 (IRDye 800

Zeiss Axioscope: Quantitative Light Microscopy Core Facility - UT Southwestern, Dallas, Texas Zeiss Axioscope (NL7.110D) Zeiss Axioscop upright microscope with fluorescence, DIC & polarization Filters for DAPI, FITC, TxRed and Cy5 Lumenera color digital camera and software for image acquisition Sign